Aptasensor for Impedimetric Detection of LysozymeĮce Eksin, Huseyin Senturk, and Arzum Erdemġ6. Nguyen Quang N, Perret G, Duconge F (2016) Applications of high-throughput sequencing for in vitro selection and characterization of aptamers. Aptamer-Field-Effect Transistors for Small-Molecule Sensing in Complex Environmentsġ5. Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINTġ4. Probing of Fluorogenic RNA Aptamers via Supramolecular F rster Resonance Energy Transfer with Universal Fluorescent Nucleobase AnalogĬhristian Steinmetzger and Claudia H bartnerġ3. Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance Nico Dreymann, Anja M ller, and Marcus M. Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis Yannick Kerler, Stephan Sass, Carsten Hille, and Marcus M. Our emulsion PCR protocol is easily integrated into existing SELEX protocols, requires no special laboratory equipment, and can be performed with easily commercially available reagents. Determination of Aptamer Structure Using Circular Dichroism Spectroscopy In this chapter, we describe a protocol for emulsion PCR and subsequent DNA recovery for implementation into click-SELEX protocols using click-modified DNA. In a typical systematic evolution of ligands by exponential. Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry Sladjana Slavkovic and Philip E. emulsion polymerase chain reaction (PCR) based on the agarose droplet microfluidic technology. Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEXĨ. The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITEħ. RNA Capture-SELEX on Streptavidin Magnetic BeadsĦ. Magda Puchala, Marta Radzińska, Joanna Guzdek, Agnieszka Sok-Grochowska, Aleksandra Adamowicz-Skrzypkowska, Pawel Pięta, Przemyslaw Jurek, and Monika Czarneckaĥ. Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction Moujab Choukeife, Anna Jonczyk, and G nter MayerĤ. Implementation of Emulsion PCR for Amplification of Click-Modified DNA during SELEX Nicole Williams, Rutika Patel, and Prabodhika Mallikaratchyģ. Discovery of Aptamers against Cell Surface Markers Using Ligand-Guided Selection Automated ssDNA SELEX Using Histidine Tagged Target ProteinsĢ.
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